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Basic laboratory equipments used in microbiology pdf

Basic laboratory equipments used in microbiology pdf

JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser. Log out. Sartorius Corp. Labconco Corp. Arrayit Corporation Beckman Coulter, Inc. Forgot Password? Balances Analytical, precision and top-loading balances and accessories by Sartorius. Select models available for immediate shipment.

basic laboratory equipments used in microbiology pdf

Calorimeters Space-saving, low-cost calorimeters determine gross calorific values of liquid and solid samples; capable of measuring in adiabatic, isoperibolic, dynamic and time-controlled modes. Cryogenics Cryogenic systems and dewars for long-term storage of blood, infectious disease and cell culture samples.

Dispersers IKA hand-held and stand-mounted dispersers for homogenization, emulsification and suspension. Select models, reagents, kits stains and dyes in stock for immediate shipment.

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Evaporators IKA and Labconco evaporators and concentrators efficiently accelerate the evaporation process for high-yield production of distillates from a range of solvents.

Gel Imaging Systems Find and compare pricing on compatible Gel Imaging Systems for use in molecular biology labs imaging and documenting nucleic acids and proteins. Select models disperse nanoparticles to create chemical emulsions. Hot Plates Analog and digital hotplates by Thermo Fisher and Benchmark Scientific in stirring, magnetic and explosion-proof models. CO2, refrigerated, B. D, drosophila and microbiological models available. Test chambers from Binder for drug stability studies.

Lab Consumables Lab consumables including microplates, centrifuge tubes, purification and isolation kits, inoculating loops and biohazard bags. Lyophilizers Labconco FreeZone Lyophilizers freeze dry samples for transport and long-term storage.

Mills Benchtop lab mills from IKA for grinding, cutting and homogenization of hard, brittle, fibrous or soft materials.

Basic Laboratory Glassware and Equipment

Moisture Analyzers Moisture Analyzers from Sartorius for analysis of food, pharmaceutical and environmental samples. Overhead Stirrers IKA Overhead Stirrers perform a range of mixing tasks for volumes from 15 to L; available with a wide range of stirring elements. Pipettes Mechanical and electronic pipettes from Sartorius Biohit. Single-channel and multi-channel pipettors available for immediate shipment.

Visit TerraUniversal. Lab instruments, equipment and supplies from trusted brands Laboratory-Equipment provides innovative, quality scientific research equipment from top-tier suppliers, including Thermo Fisher, Labconco, Sartorius, PerkinElmer, IKA, Sheldon, Binder and Benchmark Scientific. You need at least 2 products to compare Compare Clear All.

Compare Products.A set of micropipettes. Colorimeter spectrophotometer. Antique light microscope. From Wikipedia, the free encyclopedia. This is a list of instruments used in general in laboratoriesincluding: Biochemistry Microbiology Pharmacology [1] [2] [3] This list is incomplete ; you can help by expanding it.

A cuvette of a colorimeter. Glass Pasteur pipettes with teats. Chakraborty and Dr. Medical instruments and implants.

Dentistry General medicine General surgery.

List of Lab Instruments and Their Use

Anatomy Forensic sciences. Laboratory equipment. Stands Clamps Holders. Dean—Stark Soxhlet extractor Kipp's. Boston round Pycnometer. Cold finger Liebig. Evaporating Petri Syracuse Watch glass. Beaker Bell jar Gas syringe Vial. Analytical chemistry. Personal protective equipment PPE.

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Lab coat Face shield Respirator Rubber apron Safety shower.Microbiology laboratory is a basic laboratory in biology domain, to prepare a complete microbiology laboratory, do you know which are the microbiology laboratory essential instruments? Henan Lanphan offer you following references for some of the essential microbiology lab equipment. Clean bench is a basic essential microbiology lab equipment, microbiology cultivation is axenic cultivation, it requires a totally clean bench to provide an axenic working environment.

There are various types of incubators, it has function of creating a suitable environment for microorganism development. Thermostat water bath. It is microbiology laboratory essential instruments of a kind of temperature control device. Thermostat Water Bath of Henan Lanphan. As one of the basic microbiology laboratory essential instruments, pure water device includes pavilion distiller and water purification machine.

Essential microbiology lab equipment constant temperature drying oven is used for sterilization and drying. Microbiology laboratory essential instrumentsliquid moving machine is used to precisely get all types liquid.

Commonly used liquid measuring vessel includes measuring cylinder, transfer pipette, scale test tube, beaker, etc. Above are the introduction of microbiology laboratory essential instruments, we also need other consumable items such as alcohol burner, conical flask, measuring cylinder and so on.

Experimenter should equip microbiology laboratory essential instruments according to their needs.

basic laboratory equipments used in microbiology pdf

Clean bench Clean bench is a basic essential microbiology lab equipment, microbiology cultivation is axenic cultivation, it requires a totally clean bench to provide an axenic working environment. Incubator There are various types of incubators, it has function of creating a suitable environment for microorganism development. Thermostat water bath It is microbiology laboratory essential instruments of a kind of temperature control device.

Thermostat Water Bath of Henan Lanphan 5.

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Pure water device As one of the basic microbiology laboratory essential instruments, pure water device includes pavilion distiller and water purification machine. Constant temperature drying oven Essential microbiology lab equipment constant temperature drying oven is used for sterilization and drying.

Autoclaved sterilization pot Most laboratory material, reagent, culture medium should be strictly sterilized. Liquid-moving machine Microbiology laboratory essential instrumentsliquid moving machine is used to precisely get all types liquid.

Article Category. Contact Us. Hot Article.In the laboratory, eight main types of instruments are used:- 1. Balance 2. Centrifug e 3. Hot Air Oven 4. Incubators 5. Water Bath 6. Microscope 7. Autoclave 8. Laminar Flow. By advancement in technology to make the laboratory testings more accurate, fast, reliable and cost effective, we require these instruments with its proper care and maintenance.

Instruments used in microbiology

A balance is used to find out the mass of a substance by comparing it with known masses. These are used to weigh the chemicals accurately. Physical balance is based on the principle of moments according to which, when a body is in equilibrium, under the action of a number of forces acting on it in the same plane, the sum of the clockwise moments is equal to the sum of the anticlockwise moments.

The balance should not be loaded with a weight greater than the maximum. They are designed to accurate the sedimentation process by using centrifugal force. Centrifuge is applying centrifugal force to separate the useful component in mixtures of liquids and solids or liquids and liquids.

Centrifuge is mainly used to separate solids from liquids in suspension or separate two liquids with different density and non-homogenous liquids, for example, separate cream form milk, and also it can be used to remove liquids existed in solids, such as special speeding tubular centrifuges can separate the mixed gas content with different density, depending different density and particle size of solid particles in the liquid and different characteristics of the subsiding speed centrifuge, the sedimentation centrifuge also can classified solids according to different density and particle size.

Centrifuge is widely used in chemical, oil, food, pharmaceutical, beneficiation, coal, water treatment and shipping etc. Centrifuge has a drum rotating its axle called bowl, generally derived by motor. Suspension or emulsion is introduced to the bowl and rotate with bowl with the same speed, eject separately under the centrifugal force.

Usually, high separation speed, high separation ratio. The principle of centrifuge is divided to centrifugal filtering and centrifugal sedimentation. Centrifugal filtering is made suspension become filtrate under the centrifugal force. And the centrifugal sedimentation is applied different density to separate suspension and emulsion and realize liquid-solid or liquid-liquid separation. Care and Maintenance :.

Before the centrifugation the centrifuge tubes should be balanced properly. All the spells should be cleaned immediately. This kind of dry heat sterilization is recommended when it is undesirable that steam make contact with the material to be sterilized.

Even the kitchen oven can be used. Light bulbs heat air in the bottom part of the incubator. The air passes over a container with evaporating water, so that its humidity increases.

The warm, humid air then flows upwards chimney effect into the compartment. A thermostat in an exit hole compares the air temperature with the desired temperature. If it is too high, the light bulbs will be switched off; if it is too low, the bulbs will be switched on. The incubator operates with a thermostat that keeps the temperature of the air at a constant, adjustable value. They are used for growing microorganisms on various culture media; it must be properly cleaned before and after uses.

It is used to carry out various chemical reactions at specific temperature. Depending upon the requirement of an experiment. To do this scientists and cooks often submerge the vessel needing this controlled environment into a bath of water at a controlled temperature.

The laboratory water bath is slightly more complex than the kitchen version the double boiler as the temperature can be controlled through sensors to within a degree of the desired temperature.Video: Introduction to cell culture This video provides an overview of the basic equipment used in cell culture and proper laboratory set-up.

Guidance on how to work safely and aseptically in a cell culture hood is introduced and demonstrated. A cell culture laboratory should have storage areas for liquids such as media and reagents, for chemicals such as drugs and antibiotics, for consumables such as disposable pipettes, culture vessels, and gloves, for glassware such as media bottles and glass pipettes, for specialized equipment, and for tissues and cells. Glassware, plastics, and specilized equipment can be stored at ambient temperature on shelves and in drawers; however, it is important to store all media, reagents, and chemicals according to the instructions on the label.

Some media, reagents, and chemicals are sensitive to light; while their normal laboratory use under lighted conditions is tolerated, they should be stored in the dark or wrapped in aluminum foil when not in use. A cell counter is essential for quantitative growth kinetics, and a great advantage when more than two or three cell lines are cultured in the laboratory.

Now with fluorescence capability—brightfield and two optional fluorescence channels—researchers can count cells, monitor fluorescent protein expression, and measure cell viability.

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Note: You clicked on an external link, which has been disabled in order to keep your shopping session open. Search Thermo Fisher Scientific. Search All. See Navigation. The purpose of the incubator is to provide the appropriate environment for cell growth.

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Stainless steel incubators allow easy cleaning and provide corrosion protection, especially if humid air is required for incubation. Although the requirement for aseptic conditions in a cell culture incubator is not as stringent as that in a cell culture hood, frequent cleaning of the incubator is essential to avoid contamination of cell cultures.

Types of Incubators There are two basic types of incubators, dry incubators and humid CO 2 incubators. Dry incubators are more economical, but require the cell cultures to be incubated in sealed flasks to prevent evaporation. Placing a water dish in a dry incubator can provide some humidity, but they do not allow precise control of atmospheric conditions in the incubator.

Humid CO 2 incubators are more expensive, but allow superior control of culture conditions. They can be used to incubate cells cultured in Petri dishes or multiwell plates, which require a controlled atmosphere of high humidity and increased CO 2 tension. For larger laboratories, a cold room restricted to cell culture is more appropriate.

basic laboratory equipments used in microbiology pdf

Make sure that the refrigerator or the cold room is cleaned regularly to avoid contamination. A domestic freezer is a cheaper alternative to a laboratory freezer. While most reagents can withstand temperature oscillations in an autodefrost i.A modern microbiology laboratory should be furnished with the following equipment. Liquid substances, such as prepared media and saline solutions cannot be sterilized in oven, as they lose water due to evaporation.

An oven Figure 3. The thermostat dial reading is approximate and the exact temperature is read by introducing a thermometer into the oven or on a built-in L-shaped thermometer. In a modern oven Figure 3.

Time is set by a digital timer. The required temperature is set. After the oven attains the set temperature, the required time of sterilization is set on the timer. The oven switches off automatically after the set time. The oven is opened, only after its temperature comes down near to room temperature. Autoclave is the nucleus of a microbiology laboratory. It has the same working principle as a domestic pressure cooker.

This temperature is sufficient to kill only the non-spore formers, but it is difficult to kill the spore-forming bacteria at this temperature, as they escape by forming heat resistant spores. It takes very long time to kill the spores at this temperature. This high temperature is required to kill all the bacteria including the heat resistant spore-formers. Steam temperature increases with increase in steam pressure Table 3.

In operating a standard vertical autoclave, Figure 3. If water is too less, the bottom of the autoclave gets dried during heating and further heating damages it. If it has in-built water heating element, Figure 3. On the other hand, if there is too much water, it takes long time to reach the required temperature. The materials to be sterilized are covered with craft paper and arranged on an aluminium or wooden frame kept on the bottom of the autoclave, otherwise if the materials remain half-submerged or floating, they tumble during boiling and water may enter.

The autoclave is closed perfectly airtight only keeping the steam release valve open. Then, it is heated over flame or by the in-built heating element. Air inside the autoclave should be allowed to escape completely through this valve. When water vapour is seen to escape through the valve, it is closed.

Temperature and pressure inside goes on increasing. The pressure increase is observed on the pressure dial. The required time is considered from the point, when the required temperature-pressure is attained. Once required temperature-pressure is attained, it is maintained by controlling the heating source. After the specified time 15 minutesheating is discontinued and steam release valve slightly opened.

If fully opened immediately, due to sudden fall in pressure, liquids may spill out from the containers. Gradually, the steam release is opened more and more, so as to allow all steam to escape. The autoclave is opened only after the pressure drops back to normal atmospheric pressure 0 psi.

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The autoclave should never be opened, when there is still pressure inside. The hot sterilized materials are removed by holding them with a piece of clean cloth or asbestos- coated hand gloves. In case of a steam-jacketed horizontal autoclave, a boiler produces the steam Figure 3. It is released at a designated pressure, into the outer chamber jacket. Air is allowed to escape and then its steam release valve is closed.

The hot jacket heats the inner chamber, thereby heating the materials to be sterilised. This prevents condensation of steam on the materials.To get the best possible experience using our website, we recommend that you upgrade to latest version of this browser or install another web browser. Network with colleagues and access the latest research in your field. Find a chemistry community of interest and connect on a local and global level.

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basic laboratory equipments used in microbiology pdf

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This page details common laboratory equipment and their appropriate use. Knowing the proper use will help ensure safe laboratory practices. Used to transfer liquids or fine-grained materials into containers with small openings.

Also used for filtration. Used to hold or clamp laboratory glassware and other equipment in place, so it does not fall down or come apart.

Basic Laboratory Equipment & Supplies

Used to measure small amounts of liquid very accurately. Never pipet by mouth! Use pipetting aids. Used to support a container, such as a beaker, on a ring stand while it is being heated.

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